The Queen can be found in random locations in Dead Rising 2. Certain zombies act as hosts to fully-developed queens, and can be seen clawing at the backs of their necks. When Chuck encounters a host, it will belch blood on Chuck, causing him to cough and gag. Once the host is killed, the queen will dislodge itself and fly around the Chuck's general proximity. The queen can be captured and killed in the middle of a large crowd to dispatch many zombies at once. Once the queen is smashed, any zombie within 10 feet of Chuck will begin to have spastic convulsions for a few seconds before having their head explode.
Later in the game, when the Gas Zombies start to appear, the queens can also be obtained from then, but these ones will try to escape by fleeing to the nearest vent.
Pressing the attack button while standing still is considerably faster in Dead Rising 2 than it is in Dead Rising.
Just like in the original Dead Rising, the jars used to catch the Queens seemingly appear out of nowhere.
Before Chuck crushes the first Queen in Case Zero, he pauses a moment to watch the zombies closing in on him, which suggests he is encountered the wasps before, and knows their effects on the zombified populace; he also does this in Dead Rising 2.